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Objective:To investigate the diagnostic value of renal injury molecule-1 (KIM-1) and β2-microglobulin (β2-MG) concentration in hypertensive disorder of pregnancy (HDP) complicated with early renal injury.Methods:From July 2017 to October 2018, the clinical data of 56 pregnant women with HDP complicated with renal injury and 56 normal pregnant women in the same period in Xi′an Central Hospital were analyzed retrospectively.The pregnant women with HDP complicated with renal injury were the observation group and the 56 normal pregnant women were the control group.The difference of serum creatine (SCr), KIM-1, β2-MG concentration between the two groups at 30, 32, 34 and 36 weeks of gestation was compared, and the value of KIM-1, β2-MG combined diagnosis of early renal injury in HDP was analyzed.Results:At 30, 32, 34 and 36 weeks of gestation, Kim-1, β2-MG and SCr in the observation group increased significantly (Kim-1: Fintra-group=11.234, β2-MG: Fintra-group=12.852, SCr: Fintra-group=8.308; all P<0.001). In the observation group, SCr(30 weeks, 32 weeks, 34 weeks, 36 weeks: (75.35±10.32), (75.41±10.35), (77.02±10.50), (87.78±10.64) μmol/L), Kim-1 (30 weeks, 32 weeks, 34 weeks, 36 weeks: (5.68±1.50), (7.56±1.52), (8.25±1.62), (9.65±1.76) ng/L), β2-MG (30 weeks, 32 weeks, 34 weeks, 36 weeks: (0.26±0.07), (0.29±0.08), (0.75±0.29), (1.02±0.38) ng/L) were all higher than those of control group SCr(30 weeks, 32 weeks, 34 weeks, 36 weeks: (74.37±10.34), (74.43±10.40), (75.10±12.52), (76.80±11.66) μmol/L), Kim-1 (30 weeksL, 32 weeks, 34 weeks, 36 weeks: (5.31±1.75), (5.36±1.77), (5.39±1.60), (5.41±1.70) ng/L), β2-MG (30 weeks, 32 weeks, 34 weeks, 36 weeks: (0.25±0.07), (0.26±0.09), (0.28±0.08), (0.27±0.08) mg/L), the difference was statistically significant (SCr: Fintergroup=10.254, Kim-1: Fintergroup=16.352, β2-MG: Fintergroup=18.221; all P<0.001). There were interaction between the two groups at different time(SCr: Finteraction=15.632, Kim-1: Finteraction=25.645, β2-MG: Finteraction=34.251; all P<0.001). The sensitivity, specificity and accuracy of Kim-1 combined with β2-MG were 96.43% (54/56), 94.64% (53/56) and 95.54% (107/112), respectively.It was significantly better than Kim-1 alone (88.24% (45/51), 92.86% (52/56), 86.61% (97/112)), β2-MG alone (81.13% (43/53), 78.57% (44/56), 77.68% (87/112)), the difference was statistically significant (all P<0.05). Conclusion:The serum levels of KIM-1 and β2-MG in pregnant women with early renal injury of HDP are significantly increased, which can be used as a reliable indicator for prenatal monitoring and screening of early renal injury, and the combined detection can improve the accuracy of diagnosis.
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AIM: To investigate the protective effect of microRNA-218 (miR-218) silencing on kidney tissue of streptozotocin (STZ)-induced diabetic nephropathy rats and the potential mechanism.METHODS: The diabetic rat model was established by a single intraperitoneal injection of STZ (50 mg/kg).Meanwhile, the miR-218 short hairpin RNA (shRNA) lentiviral vector was constructed.The Sprague-Dawley rats were randomly divided into 4 groups: healthy control group, diabetes group, empty vector group and miR-218-shRNA group.The blood glucose, 24 h urinary protein, serum creatinine (SCr) and blood urea nitrogen (BUN) in the rats at different time points (4, 8 and 12 weeks) were measured by an automated analyzer.The expression of miR-218 was detected by RT-qPCR, while the expression of heme oxygenase-1 (HO-1), nephrin and p38 mitogen-activated protein kinase (p38 MAPK) at mRNA and protein levels in the kidney tissues was determined by RT-qPCR and Western blot.The caspase-3 activity was detected by caspase-3 activity assay kit, and the cell apoptosis of the kidney tissues was analyzed by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL).RESULTS: Compared with healthy control group, the expression of miR-218 was significantly increased in STZ-treated rats.Meanwhile, the concentrations of blood glucose, 24 h urinary protein, SCr and BUN were significantly increased in STZ-treated rats (P<0.05).The mRNA and protein expression of HO-1 and nephrin was significantly decreased, while the level of phosphorylated p38 MAPK was significantly increased in STZ-treated rats.In addition, the activity of caspase-3 was also significantly increased in STZ-treated rats.When the model rats were infected with miR-218-shRNA, the expression of miR-218 was significantly decreased and the above effects were markedly reversed.Furthermore, TUNEL results showed that compared with diabetic group and empty vector group, miR-218 silencing significantly attenuated the cell apoptosis in the kidney tissues in miR-218-shRNA group.CONCLUSION: miR-218 is involved in the kidney injury in diabetic rats, and silencing of miR-218 by lentiviral vector-mediated miR-218-shRNA transfection effectively inhibits kidney cell apoptosis, suggesting that miR-218 is a potential target for the treatment of diabetic nephropathy.
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<p><b>OBJECTIVE</b>To study the molecular mechanism of Yangjing Zhongyu Decoction (YZD) n-butanol extracts (ZDC) and ethyl acetate extracts (YSYZ) in reducing androgen in porcine granulose cells by mitogen-activated protein kinase (MAPK) pathway.</p><p><b>METHODS</b>Porcine granulose cells were isolated and cultured. They were inoculated by MAPK inhibitor PD98059 at different concentrations, and then they were divided into the blank control group (0), 1, 3, 10, and 25 micromol/L groups. After 24-h culture the cytochrome P450c17a (CYP17) mRNA expression level was detected using Real-time fluorescent quantitative PCR. Contents of androgen (testosterone) in the supernate were detected using RIA and optimal PD98059 concentration screened. After intervened by 10 micromol/L PD98059 for 24 h, the culture solution was intervened by effective ingredients of with or without YZD or YSYZ at various concentrations (0, 1 , 5, 25, 50 mg/mL) at various time points (3, 6, 18, 24 h). Expression levels of p-ERK1/2, c-Fos and CYP17 were detected by Western blot. Testosterone content in the supernate was determined by radioimmunoassay (RIA).</p><p><b>RESULTS</b>Ten pLmol/L PD98059 could obviously decrease p-ERK1/2 protein expression and increase CYP17 mRMA expression, and elevate testosterone content in the supernate (P < 0.05). ZDC and YSYZ at 25 ng/mL could increase p-ERK1/2 protein expression and c-Fos levels, and reduce CYP17 protein expression, and lower testosterone content in the supernate after 6-h intervention (P < 0.01).</p><p><b>CONCLUSION</b>Effective ingredients of YZD could reduce androgen production in porcine granulose cells through increasing activities of MAPK.</p>
Subject(s)
Animals , Female , Androgens , Drugs, Chinese Herbal , Pharmacology , Flavonoids , Granulosa Cells , Metabolism , Mitogen-Activated Protein Kinase 3 , Metabolism , Mitogen-Activated Protein Kinases , Metabolism , RNA, Messenger , SwineABSTRACT
The wild Pinellia ternata resource was surveyed by field investigation, visiting surveys combine with literature study and changing factors of the wild P. ternata in the area of Huanghuai Plain were analyzed. The results indicated that Huanghuai Plain wild P. ternata resources were scarce and nearly extinct. The chemical farming methods in Huanghuai Plain have endangered the survival of the wild P. ternata.